Dr. Adrienne Hollister

Research interests:

Trace metals and organic matter in the Amazon and Pará River estuary and mixing plume, specifically:

  • Copper (Cu) speciation and Cu-binding organic ligands along the estuary salinity gradient
  • Nickel (Ni) and cobalt (Co) speciation and concentrations in surface and depth profiles throughout the estuary
  • Fluxes of trace metals (Al, Mn, Fe, Co, Ni, Cu, Zn, Cd, Pb) from the estuary into the Atlantic Ocean

 Project description and background:

Broadly, I am interested in understanding trace metal biogeochemistry in the oceans. Trace metals are essential for life, but are present in nano- or picomolar concentrations in the ocean. For my Master’s thesis (Aug 2016 – Mar 2019), I worked under Dr. Kristen Buck at the University of South Florida (USF), USA. Here, I researched the regeneration of the trace metals (Mn, Fe, Co, Ni, Cu, Zn, Cd, and Pb) and macronutrients (N, P, Si) from phytoplankton assemblages. In April, 2019, I entered a Ph.D. program under Prof. Dr. Andrea Koschinsky at Jacobs University Bremen, where I have continued to research on trace metals biogeochemistry – this time looking at trace metals in the Amazon River estuary!

The Amazon River is the largest on earth and makes up 15-20% of the global freshwater discharge. It is therefore an important source of dissolved trace metals and organic matter to the Atlantic Ocean. In the estuary, the larger Amazon River also mixes with the smaller Pará River to the south (earth’s 5th largest river), and groundwater from a large mangrove belt from the southeast serves as an additional source of trace metals and organic matter. Together, these waters mix and form a plume that ages as it flows north, mixing with seawater in the Atlantic Ocean. Trace metals are lost or gained during estuary mixing due to factors such as adsorption/desorption to particles, colloidal flocculation, benthic exchange, or biological activity. However, recent trace metal data from the Amazon and Pará estuary are sparse.

I joined the project, “Development of a consistent thermodynamic model of trace element – organic matter interaction in the ocean,” where I have been working to analyse trace metal and organic ligand samples collected previously on the Amazon-GEOTRACES cruise M147. In this project, I have also been working closely with Dr. Martha Gledhill at GEOMAR Helmholz Centre for Ocean Research. My analytical work has focused on (1) quantification and size fractionation of trace metals (Mn, Fe, Co, Ni, Cu, Zn, Cd, Pb) in surface and depth samples throughout the estuary and (2) analysis of Cu-organic matter speciation in these samples.

In addition to my work on samples previously collected on M147, I had the opportunity to go to the Amazon myself on cruise M174 (Apr-May 2021) (blog: https://www.io-warnemuende.de/rv-meteor-m174-2021.html). Here, I worked with another PhD student colleague to collect trace metal samples, as well as conducting an incubation experiment to measure Cu-ligand production by phytoplankton. In addition, I participated on the North Sea cruise M169 (Dec 2020) (blog: https://www.jacobs-university.de/blog-post-m169). This served as the basis for a bachelor student thesis on the analysis of platinum in the North Sea, which I co-supervised. Finally, I had the opportunity to participate in a week-long GEOTRACES summer school in Cadiz Spain (Aug 2019) (https://geotraces.uca.es/), where I learned first-hand about GEOTRACES trace-metal clean protocol with 40 other students from a total of 19 countries aboard the vessel Intermares. In the future, I hope to return to the Amazon Estuary during a period of low-discharge, which has been already approved as a GEOTRACES cruise. Since both previous Amazon cruises (M147 and M174) took place during the high river discharge period, this cruise will help complete the picture of trace metal cycling in this region.


GEOMAR: https://www.geomar.de/en/

Oldenburg ICBM: https://uol.de/en/icbm/

Ongoing projects:

2019- 2022 Thermodynamic Model (DFG)

Development of a consistent thermodynamic model of trace element – organic matter interaction in the ocean

2016 – 2020 Amazon-GEOTRACES (DFG)

RV Meteor (M147) research cruise to Amazon River estuary, May 2018

Previous projects:

2016 – 2019: “Regeneration of macronutrients and trace metals during phytoplankton decay: an experimental study” (Advisor: Dr. Kristen Buck, University of South Florida)

R/V W. T. Hogarth research cruise, Eastern Gulf of Mexico and West Florida shelf, Mar 2018

News interview about the cruise: https://www.usf.edu/marine-science/news/2018/usf-students-gain-valuable-experience-on-floating-lab.aspx

2018 – 2019: Harmful Algal Bloom (HAB) Mitigation Project (PI: Dr. Donald Anderson, Woods Hole Oceanographic Institute)

Science outreach involvement:

2019: Jacobs University VDI summer camp: https://www.jacobs-university.de/news/using-holidays-study-summer-camp-jacobs-university

OCG lab blogs

2018: https://www.usf.edu/marine-science/news/2018/iron-bubbles-oil-spill-troubles.aspx

2017: OCG in the lab: Ocean Chemistry (not available online anymore)

St. Pete Science Fest: https://www.stpetescifest.org/


de Carvalho, L. M.; Hollister, A.P., Trindade, C.; Gledhill, M. and Koschinsky, A. (2021) Distribution and size fractionation of nickel and cobalt species along the Amazon estuary and mixing plume. Marine Chemistry 236: 104019https://doi.org/10.1016/j.marchem.2021.104019

Hollister, A. P.; Whitby, H.; Seidel, M.; Lodeiro, P.; Gledhill, M.; Koschinsky, A. (2021) Dissolved concentrations and organic speciation of copper in the Amazone estuary and mixing plume. Marine Chemistry 234: 104005. doi: 10.1016/j.marchem.2021.104005

Hollister, A. P.; Kerr, M.; Malki, K.; Muhlbach, E.; Robert, M.; Tilney, C. L.; Breitbard, M.; Hubbard, K. A. and Buck, K. N. (2020) Regeneration of macronutrients and trace metals during phytoplankton decay: an experimental study. Limnology & Oceanography 1-20. doi: 10.1002/lno.11429

For coauthored publications prior to my Master’s work, refer to my Google Scholar profile: http://tinyurl.com/Hollister-Scholar


Conference presentations:

  • Hollister, A. P., Koschinsky, A., Gledhill, M. Dissolved concentrations and organic speciation of Cu in the Amazon River estuary and mixing plume. Talk. Ocean Sciences Meeting, 2020, San Diego, USA.
  • Hollister, A.P., Buck, K. N., Hubbard, K., Robert, M., Tilney, C. L., Schlosser, C., Gledhill, M., Koschinsky, A. An Experimental Study of Trace Metal Regeneration and Implications for the Amazon River Estuary. Poster. Goldschmidt, 2019, Barcelona, Spain.
  • Hollister, A. P., Palmer, K., Robert, M., Tilney, C., Breitbart, M., Djurhuus, A., Hubbard, K., Buck, K. Remineralization of trace metals and macronutrients from natural phytoplankton assemblages, pseudo-nitzschia dolorosa and Karenia brevis. Aquatic Sciences meeting (ASLO), 2019, San Juan, PR, USA.
  • Hollister, A. P., Buck, K. N. Mapping Marine Trace Metal and Macronutrient Remineralization Following a Phytoplankton Bloom. Poster. Southeastern Biogeochemistry Symposium,  2018, Tallahassee, FL, USA.
  • Hollister, A. P., Buck, K. N. Riding the Ferrous Wheel: A Study in Marine Iron Remineralization. Poster. American Chemical Society (ACS) Florida Section Meeting and Exposition (FAME), 2017, Tampa, FL, USA.